Louise Giam, Stanford University
Conference: Society for Neuroscience 2017, Washington DC
Abstract Title: Developmental characterization of human induced neurons
Abstract: Our lab recently reported that a single transcription factor (Neurogenin-2) can drive the differentiation of human embryonic stem cells into functional induced neurons (iNs) over several weeks. These iNs express synaptic markers at both transcript and protein levels and exhibit electrophysiological properties of excitatory neurons. This reduced system presents many opportunities, but in order to be useful for genetic screens and manipulations, we must understand the transcriptomic and proteomic profiles of these neurons in both immature and mature states. We developed strategies to culture pure iNs that were functionally equivalent to previous iNs grown on mouse glial cells in order to label proteins quantitatively using stable isotope labeling of amino acids (SILAC) and conduct RNAseq.